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排序方式: 共有59条查询结果,搜索用时 31 毫秒
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C. Höller S. G. Micha S. Schulz W. Francke J. A. Pickett 《Cellular and molecular life sciences : CMLS》1994,50(2):182-185
Females of the parasitic waspAphidius uzbekistanicus, a specialist aphid primary parasitoid, react to the presence of their specialist hyperparasitoid enemyAlloxysta victrix by leaving the area. The wasps leave to increase their reproductive success, because only their offspring, but not the wasps themselves, are threatened by enemy attack. Furthermore, this dispersal is elicited through the action of volatile chemical cues produced by the hyperparasitoids. We provide evidence for 6-methyl-5-hepten-2-one being one of the volatiles eliciting dispersal. The tendency of the wasps to leave areas which are being colonized by their enemies reduces their efficiency as aphid antagonists. 相似文献
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Witt H Sahin-Tóth M Landt O Chen JM Kähne T Drenth JP Kukor Z Szepessy E Halangk W Dahm S Rohde K Schulz HU Le Maréchal C Akar N Ammann RW Truninger K Bargetzi M Bhatia E Castellani C Cavestro GM Cerny M Destro-Bisol G Spedini G Eiberg H Jansen JB Koudova M Rausova E Macek M Malats N Real FX Menzel HJ Moral P Galavotti R Pignatti PF Rickards O Spicak J Zarnescu NO Böck W Gress TM Friess H Ockenga J Schmidt H Pfützer R Löhr M Simon P Weiss FU Lerch MM Teich N Keim V Berg T Wiedenmann B Luck W 《Nature genetics》2006,38(6):668-673
Chronic pancreatitis is a common inflammatory disease of the pancreas. Mutations in the genes encoding cationic trypsinogen (PRSS1) and the pancreatic secretory trypsin inhibitor (SPINK1) are associated with chronic pancreatitis. Because increased proteolytic activity owing to mutated PRSS1 enhances the risk for chronic pancreatitis, mutations in the gene encoding anionic trypsinogen (PRSS2) may also predispose to disease. Here we analyzed PRSS2 in individuals with chronic pancreatitis and controls and found, to our surprise, that a variant of codon 191 (G191R) is overrepresented in control subjects: G191R was present in 220/6,459 (3.4%) controls but in only 32/2,466 (1.3%) affected individuals (odds ratio 0.37; P = 1.1 x 10(-8)). Upon activation by enterokinase or trypsin, purified recombinant G191R protein showed a complete loss of trypsin activity owing to the introduction of a new tryptic cleavage site that renders the enzyme hypersensitive to autocatalytic proteolysis. In conclusion, the G191R variant of PRSS2 mitigates intrapancreatic trypsin activity and thereby protects against chronic pancreatitis. 相似文献
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Seibert MM Ekeberg T Maia FR Svenda M Andreasson J Jönsson O Odić D Iwan B Rocker A Westphal D Hantke M DePonte DP Barty A Schulz J Gumprecht L Coppola N Aquila A Liang M White TA Martin A Caleman C Stern S Abergel C Seltzer V Claverie JM Bostedt C Bozek JD Boutet S Miahnahri AA Messerschmidt M Krzywinski J Williams G Hodgson KO Bogan MJ Hampton CY Sierra RG Starodub D Andersson I Bajt S Barthelmess M Spence JC Fromme P Weierstall U Kirian R Hunter M Doak RB Marchesini S Hau-Riege SP Frank M 《Nature》2011,470(7332):78-81
X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions. Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma. The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval. Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a non-crystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source. Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000?K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies. 相似文献
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We report a population of the mussel the black sandshell ( Ligumia recta [Lamarck, 1819]) from the Carrot River in Saskatchewan, far north of the species’ known range in southern Manitoba and northwestern Ontario. Ligumia recta is reported to be declining substantially in its abundance and range, and this occurrence provides valuable information for the construction of a species status report in Canada. 相似文献
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Systemic Practice and Action Research - The purpose of this paper is to detail a systematic and systemic approach to capturing and embedding multiple customers’ expectations into a framework... 相似文献
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Loh ND Hampton CY Martin AV Starodub D Sierra RG Barty A Aquila A Schulz J Lomb L Steinbrener J Shoeman RL Kassemeyer S Bostedt C Bozek J Epp SW Erk B Hartmann R Rolles D Rudenko A Rudek B Foucar L Kimmel N Weidenspointner G Hauser G Holl P Pedersoli E Liang M Hunter MS Hunter MM Gumprecht L Coppola N Wunderer C Graafsma H Maia FR Ekeberg T Hantke M Fleckenstein H Hirsemann H Nass K White TA Tobias HJ Farquar GR Benner WH Hau-Riege SP Reich C Hartmann A Soltau H Marchesini S Bajt S 《Nature》2012,486(7404):513-517
The morphology of micrometre-size particulate matter is of critical importance in fields ranging from toxicology to climate science, yet these properties are surprisingly difficult to measure in the particles' native environment. Electron microscopy requires collection of particles on a substrate; visible light scattering provides insufficient resolution; and X-ray synchrotron studies have been limited to ensembles of particles. Here we demonstrate an in situ method for imaging individual sub-micrometre particles to nanometre resolution in their native environment, using intense, coherent X-ray pulses from the Linac Coherent Light Source free-electron laser. We introduced individual aerosol particles into the pulsed X-ray beam, which is sufficiently intense that diffraction from individual particles can be measured for morphological analysis. At the same time, ion fragments ejected from the beam were analysed using mass spectrometry, to determine the composition of single aerosol particles. Our results show the extent of internal dilation symmetry of individual soot particles subject to non-equilibrium aggregation, and the surprisingly large variability in their fractal dimensions. More broadly, our methods can be extended to resolve both static and dynamic morphology of general ensembles of disordered particles. Such general morphology has implications in topics such as solvent accessibilities in proteins, vibrational energy transfer by the hydrodynamic interaction of amino acids, and large-scale production of nanoscale structures by flame synthesis. 相似文献
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To understand the physical processes that occur in nature we need to obtain a solid concept about the 'fundamental' forces acting between pairs of elementary particles. It is also necessary to describe the temporal and spatial evolution of many mutually interacting particles under the influence of these forces. This latter step, known as the few-body problem, remains an important unsolved problem in physics. Experiments involving atomic collisions represent a useful testing ground for studying the few-body problem. For the single ionization of a helium atom by charged particle impact, kinematically complete experiments have been performed since 1969 (ref. 7). The theoretical analysis of such experiments was thought to yield a complete picture of the basic features of the collision process, at least for large collision energies. These conclusions are, however, almost exclusively based on studies of restricted electron-emission geometries. Here, we report three-dimensional images of the complete electron emission pattern for the single ionization of helium by the impact of C6+ ions of energy 100 MeV per a.m.u. (a four-body system) and observe features that have not been predicted by any published theoretical model. We propose a higher-order ionization mechanism, involving the interaction between the projectile and the target nucleus, to explain these features. 相似文献
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